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sfGFP熒光蛋白的激發波長和發射波長

更新時間:2022-10-11   點擊次數:2098次

                                                                                                                            sfGFP熒光蛋白的激發波長和發射波長


sfGFP的激發波長主峰在488nm,發射波長主峰在507nm。

上海路陽儀器有限公司現貨銷售進口便攜式熒光蛋白激發光源,又叫熒光蛋白觀察燈、GFP激發光源,這個便攜式光源能夠激發GFPEGFPBFPCFPYFPRFPDsredmcherry等各種熒光蛋白的熒光,常備現貨的有綠色熒光蛋白、紅色熒光蛋白、黃色熒光蛋白激發光源咨

常用的報告基因包括:lacZEgfp zsGreenEYFPDsRed (DsRed2)mRFPmCherryHuCD2Thy1.1HA tagFLAG tagLuciferase等。zsGreenEGFP, EYFP都是綠色熒光。EGFPEYFP很難區分開。zsGreen是一個比較穩定的綠色熒光蛋白。DsRedTdTomato都是紅色熒光。TdTomato是一個信號非常強的熒光蛋白,對細胞/小鼠也沒有明顯的毒性。mRFPmCherry是兩個非常好的熒光蛋白,需要530nm的激發光,所以可以用LUYOR-3415RG熒光蛋白觀察燈激發,能夠用肉眼觀察到熒光蛋白在小鼠上的表達。

上海路陽儀器有限公司銷售美國路陽便攜式熒光蛋白激發光源、熒光手電筒,能夠激發各種熒光蛋白在動植物上的轉基因表達,如果您要觀察GFP熒光蛋白在植物葉片、愈傷、種子、根系的轉基因表達。上海路陽提供樣機免費試用,滿意付款。我們還在北京、鄭州、海口、武漢、哈爾濱設有代理商,可以提供上門演示。詳情請惦記店鋪查詢

sfGFP--Superfolder GFP

Molecular engineering of the green fluorescent protein (GFP) into a robust and stable variant named Superfolder GFP (sfGFP) has revolutionized the field of biosensor development and the use of fluorescent markers in diverse area of biology. sfGFP-based self-associating bipartite split-FP systems have been widely exploited to monitor soluble expression in vitro, localization, and trafficking of proteins in cellulo. A more recent class of split-FP variants, named ? tripartite ? split-FP, that rely on the self-assembly of three GFP fragments, is particularly well suited for the detection of protein-protein interactions. In this review, we describe the different steps and evolutions that have led to the diversification of superfolder and split-FP reporter systems, and we report an update of their applications in various areas of biology, from structural biology to cell biology.

What is Superfolder GFP?

Superfolder GFP is a basic (constitutively fluorescent) green fluorescent protein published in 2005, derived from Aequorea victoria. It is reported to be a very rapidly-maturing weak dimer. +.


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